Characterization of bacterial strain Lactobacillus plantarum 1K isolated from Slavonian “kulen“, as probiotic functional starter culture

U ovom je radu ispitana adhezija bakterijskoj soja L. plantarum 1K izoliranog iz kulena na tanko i debelo crijevo miša u in vitro i in vivo uvjetima. Budući da je u preliminarnim istraživanjima L. plantarum 1K pokazao značajnu inhibiciju rasta prema patogenim mikroor- ganizmima iz hrane, proizveo značajnu količinu mliječne kiseline (21,96 g/l) te zadovoljio osnovne kriterije za selekciju probiotičkog soja u in vitro uvjetima, provedena su in vivo istraživanja na pokusnim miševima. L. plantarum 1K znatno smanjuje broj bakterijskih stanica Salmonella sp. u miševa inciranih sa bakterijom Salmonella sp., te smanjuje translokaciju Salmonella sp. u jetru miševa. L. plantarum 1K je pokazao osnovne funkcionalne kriterije za izbor probiotičkih sojeva, te se kao takav može upotrijebiti kao funkcio- nalna autohtona starter kultura za fermentirane mesne proizvode.The aim of this work was to investigate adhesion ability of bacterial strain L. plantarum 1K, isolated from Slavonian “kulen”, on small and large intestine of mice in vitro and in vivo conditions. Considering that in preliminary investigation L. plantarum 1K has shown good antimicrobial activity against pathogen microorganisms from food, it has produced signicant amount of lactic acid (21,96 g/l) and satised the basic criteria for selection of probiotic strains in in vitro conditions, in vivo studies in experimental mice were carried out. L. plantarum 1K signicantly reduced the number of bacterial cells of Salmonella sp. in mice infected with bacterium Salmonella sp. and reduced the translocation of bacteria Salmonella sp. in the liver of mice. Considering that L. plantarum 1K has demonstrated the basic functional criteria for the selection of probiotic strains, so as such, it can be used as functional autochthonous starter culture for fermented meat products